ISO/AWI 20397-3

Biotechnology — Massively parallel sequencing — Part 3: General requirements and guidance for metagenomics

Scope

This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, generating and analyzing metagenomics sequence data obtained from massive parallel sequencing platforms. The specified metagenomics process includes the following stages:
a) Sampling strategy and process, including type, storage, transportation, extraction, quality;
b) Nucleic acid library preparation
c) Sequencing strategy and assessment;
d) Database construction;
e) Bioinformatics analysis and report
f) Validation and evaluation for bioinformatics pipeline, and database.
This document applies to laboratories and research organizations.