ISO/TS 19006:2016

Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line

Publication date: Jul 18, 2016

General information

Current stage: 90.20 Effective date: Jan 15, 2023

Originator: ISO

Owner: ISO/TC 229

Type: Technical Specification

ICS: 07.120

Languages: English

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Status: Published

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Scope

ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.
The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

Life cycle

NOW

PUBLISHED
ISO/TS 19006:2016
90.20 Standard under periodical review
Jan 15, 2023

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