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ISO 11063:2012

Soil quality — Method to directly extract DNA from soil samples

95.99 Withdrawal of Standard   Sep 29, 2020

General information

95.99     Sep 29, 2020

ISO

ISO/TC 190/SC 4

International Standard

13.080.30  

Scope

This International Standard specifies a method for direct extraction of DNA from soil samples to analyse the
global structure and the abundance of soil bacterial communities using PCR-based technologies. This method
is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in
organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA from soil samples provides unique insight into the richness and structure of
microbial communities which are key parameters to estimate the biodiversity of soil microbiota. Molecular
approaches based on PCR (polymerase chain reaction) amplification of soil DNA constitute a promising
domain and can contribute in the near future to the development of routine tools to monitor the microbiota of
soil environments.
Users of the method ought to be aware that although soil submitted to the DNA extraction procedure is sieved
thoroughly (2 mm mesh, procedure described in 5.1), plant residues can still remain in soil samples and, as a
result, traces of plant DNA can contaminate the soil DNA extract.

Life cycle

NOW

WITHDRAWN
ISO 11063:2012
95.99 Withdrawal of Standard
Sep 29, 2020

REVISED BY

PUBLISHED
ISO 11063:2020

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